Science & STEM

Amino Acid Quiz: Test Your Knowledge of All 20

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This amino acid quiz helps you practice the 20 amino acids, from structures and side chains to one-letter codes and properties. Keep learning with the amino acid abbreviations quiz, explore biochemistry quiz questions, or widen your practice with chemistry quiz questions.

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1How many standard amino acids are incorporated into eukaryotic proteins?
2Which amino acid has a thiol ( - SH) side chain?
3Which amino acid is achiral?
4At physiological pH (~7.4), what is the predominant form of amino acids?
5Which amino acid is aromatic?
6Which amino acid has a positively charged side chain at physiological pH?
7Which amino acid is classified as acidic at physiological pH?
8Which amino acid has a cyclic secondary amine in its side chain?
9What type of bond links amino acids in a protein?
10Which amino acid contains an imidazole side chain?
11How many chiral centers does threonine have?
12Which amino acid is essential in humans?
13What is the approximate pKa of the ?-carboxyl group of amino acids?
14What is the three-letter code for tryptophan?
15Which amino acid side chain can form hydrogen bonds via a hydroxyl group?
16Which amino acid has a guanidinium group in its side chain?
17Which amino acid is exclusively ketogenic?
18Which post-translational modification is required for stabilizing collagen's triple helix?
19Which amino acid is the precursor for gamma-aminobutyric acid (GABA)?
20Which amino acid side chain has a pKa closest to physiological pH, allowing it to act as a general acid/base in enzymes?
21What is the isoelectric point (pI) of glycine given pKa1 = 2.35 and pKa2 = 9.78?
22What stereoisomer are standard proteinogenic amino acids found in eukaryotes?
23In N-linked glycosylation, which amino acid residue is glycosylated?
24Which amino acid is the precursor for nitric oxide synthesis via nitric oxide synthase?
25Which amino acid donates one-carbon units to the folate cycle through its conversion to glycine?
Learning Goals

Study Outcomes

  1. Identify the 20 Standard Amino Acids -

    Recognize each amino acid by its full name, three-letter code, and one-letter code to solidify your foundational knowledge.

  2. Analyze Amino Acid Structures -

    Examine side chain compositions and backbone arrangements to distinguish between different amino acid structures.

  3. Classify by Polarity and Charge -

    Categorize amino acids as polar, nonpolar, acidic, or basic based on their R-group properties.

  4. Match Structures to Codes -

    Apply your understanding to link each amino acid structure with its correct three-letter and one-letter abbreviation.

  5. Apply Knowledge in a Scored Quiz -

    Engage with our amino acids quiz to test your recall and structural identification skills in a timed, scored format.

  6. Assess Your Biochemistry Proficiency -

    Receive instant feedback on your performance to pinpoint areas for review and track your progress over multiple quiz attempts.

Study Guide

Cheat Sheet

  1. Side Chain Classification & Properties -

    Understand the five main categories of side chains (nonpolar aliphatic, aromatic, polar uncharged, acidic, basic) to predict solubility and reactivity. A handy mnemonic is "GAVLIMP + FY W" for nonpolar aliphatic and aromatic amino acids. This foundational knowledge will boost your confidence during an amino acid practice session.

  2. Backbone Structure & Stereochemistry -

    Master the general amino acid backbone (amino, carboxyl, α-carbon, and R group) and L-configuration common in proteins. Visualize Fischer projections and wedge-dash drawings to ace any amino acid structure quiz question. Regular drills with a quiz amino acids tool can solidify your stereochemical recognition.

  3. Essential vs. Nonessential Amino Acids -

    Distinguish essential amino acids your body can't synthesize (like Lys, Thr, Met) from nonessential ones synthesized endogenously. The phrase "PVT TIM HaLL" helps recall the nine essentials quickly. Test yourself with a 20 amino acids quiz to ensure you've got each one memorized.

  4. Zwitterions & pKa Values -

    Know that at physiological pH (~7.4), amino acids exist as zwitterions, and their side chains ionize based on characteristic pKa values. Apply the Henderson”Hasselbalch equation to predict protonation states during titrations or buffer calculations. Practicing these calculations in an amino acid practice setting can improve your accuracy under exam conditions.

  5. Active Revision with Interactive Quizzes -

    Use focused flashcards and online quizzes like amino acids quiz modules to reinforce identification and recall. Regular timed sessions with an amino acid structure quiz platform build speed and retention through spaced repetition. Tracking your progress in a 20 amino acids quiz format motivates continued improvement and exam readiness.

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Michael HodgeEdTech Product Lead & Assessment Design SpecialistQuiz Maker
Updated Feb 23, 2026